Products

Gibson Assembly™

One of the essential technologies developed during the construction of the first synthetic cell is now available to you.

The Gibson Assembly™ is a revolutionary method developed by Daniel Gibson and colleagues at the J. Craig Venter Institute (JCVI). Synthetic Genomics Inc. sponsored this work and controls the licensing of the technology. SGI has developed in collaboration with its partners a suite of products that exploits this new technology, including Small Gene Products from Integrated DNA Technologies and Gibson Assembly™ Master Mix from New England Biolabs.

How it Works

The Gibson Assembly™ uses a one-step, isothermal approach to enable the rapid assembly of multiple DNA fragments.

Since its introduction to the life science community in 2009, the Gibson Assembly™ method has become a mainstay in many synthetic biology laboratories and has attracted interest from both the academic and commercial life sciences community due to its ease-of-use, robustness and flexibility.

Benefits

The Gibson Assembly™ enables you to:

• Assemble small and big DNA molecules, in one step, using overlapping oligonucleotides or dsDNA fragments as the starting material
• Construct genes, genetic pathways, and genomes
• Clone multiple inserts into a vector without relying on the availability of restriction sites, thereby replacing traditional methods
• Perform site-directed mutagenesis to make simultaneous DNA sequence changes including insertions, deletions, and substitutions

Licensing

SGI is licensing the Gibson Assembly™ to enable widespread use in the academic and commercial life sciences arenas. For more information on licensing contact SGI at SGI-Licensing@syntheticgenomics.com.